RNA sequencing (RNA-Seq) is a powerful technique used to analyze the transcriptome of a cell, providing insights into gene expression, splicing variations, and the presence of non-coding RNAs. This technology involves the conversion of RNA into complementary DNA (cDNA) through reverse transcription, followed by amplification and sequencing of the cDNA using high-throughput sequencing platforms. RNA-Seq enables researchers to quantify RNA levels across different conditions, identify novel transcripts, and detect gene fusions or mutations. The data generated can be analyzed to create expression profiles, which help in understanding cellular responses to various stimuli or diseases. Overall, RNA sequencing has become an essential tool in genomics, systems biology, and personalized medicine, contributing significantly to our understanding of complex biological processes.
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