RNA interference (RNAi) is a biological process in which small RNA molecules inhibit gene expression or translation by targeting specific mRNA molecules. This mechanism is crucial for regulating various cellular processes and defending against viral infections. The primary players in RNAi are small interfering RNAs (siRNAs) and microRNAs (miRNAs), which are typically 20-25 nucleotides in length.
When double-stranded RNA (dsRNA) is introduced into a cell, it is processed by an enzyme called Dicer into short fragments of siRNA. These siRNAs then incorporate into a multi-protein complex known as the RNA-induced silencing complex (RISC), where they guide the complex to complementary mRNA targets. Once bound, RISC can either cleave the mRNA, leading to its degradation, or inhibit its translation, effectively silencing the gene. This powerful tool has significant implications in gene regulation, therapeutic interventions, and biotechnology.
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